To make breakthrough progress in the molecular understanding oftranscription and its regulation
disturbances in transcriptional regulation contribute cancer, inflammation, heart disease and metabolic diseases.
Essential to be able to direct this development to form only the wanted type of cells
Gene targeting in ES cell
1. ES cell culture
2. Constructionof targeting vector
3. ES
of protein kinase A by cyclic AMP.
Phosphorylationof enzyme(activity increase or decrease) or phosphorylationof the transcription factor CREB.
Glucagon increases intracellular concentrations of calcium by a mechanism that depends upon activationof protein kinase A.
Increased intracellular calcium reinforce some cyclic AMP mediated actions of glucagon, particularly on glycogenolysis.
Ke
In this preliminary study, the variability of COX-2 overexpression in cervical premalignant lesions was assessed.
NSAIDs and COX-2 inhibitors show anti-cancer activities in many cancer cells.
Aspirin showed strongest inhibitory effects on viability and survival of YD-8 cells.
Aspirin treatment resulted in severe cell shrinkage and nuclear DNA fragmentation in YD-8 cells.
Lipopolysaccharides (LPS), also known as lipoglycans,
are large molecules consisting of a lipid and a polysaccharide
joined by a covalent bond; they are found in the outer
membrane of Gram-negative bacteria, act as endotoxins and
elicit strong immune responses in animals.
LPS is the major component of the outer membrane of
Gram-negative bacteria, contributing greatly to the structu
of free FA or ketone
→ Thus WAT is transformed to Fat burning cells
Expressionof PPARα causes leptin action by up-regulating of carnitine palmitoyl transferase(CPT)-1 and acyl CoA oxidase(ACO) which are target enzyme of FA oxidation
It can be considered on that fact that in the WAT expressionof PPARα is occurred but in the PPARα -/- condition expressionof PPARα mRNA is not occurred