HEK 293 EBNA1 cell
Freestyle medium
293 SFM II medium
Enriched medium (293 SFM II medium + 5X amino acid solution)
E.coli cell 안에서 형질전환 된 pTT
1. Freestyle midium에서 suspension 293 cell culture
2. Bioreator로 옮겨줌
3. DNA-PEI complex(pTT-EPO+PEI)로 tansfection 수행
4. 4시간 후 293 SFM II medium 첨가
5. 이틀 후 enriched medium에서 fed-batch culture
Types
MICRO-NEEDLES
SFM cantilevers
Mechanism
Apply or sense force through the displacement of a bendable beam(e.g. micro-needle, cantilevers, others)
Produces linear response over a broad range of displacement and forces
Size and shape – the two important factors that determine how a mechanical transducer interact with a single molecule or polymer
Acts molecules from a distance