shotgun...
or one of the many handguns
kept in the house.
Im going to put you down!
And when the shotgun
accidentally went off...
You asshole!
Sydney just happened to pass.
What?
Shut the fuck up!
Added to this,
the tenants turned out to be...
Fay and Arthur Barringer...
Sydneys mother and father.
When confronted
with the charge...
which took some figuring out
for the officers on the scene...
Fay
shotgunshotgun clonig은 유전자를 가진 세포의 모든 DNA를 우선 모두 추출한 다음에 이를 적당한 제한효소로 잘라서 모두 운반체에 붙이는 것입니다. 산탄식 방법이라고 하는데, 산탄이 한꺼번에 총알을 많이 쏘아 그중 하나가 명중하듯이 많은 유전자를 한꺼번에 재조합시켜서 숙주세포에 운반한 다음에
SHOTGUN comes crashing through the
cattails, wiping through frame and exiting...
??
...then ANOTHER MAN...and ANOTHER...armed with rifles,
plowing through the brush, exiting frame...
??
...and now comes KLAUS DETTERICK, a farmer one step above
shirt-tail poor, a double-barrel shotgun in the crook of his
arm. He pauses, horrified, seeing the scrap of cloth. He
pulls it loose, turns back, screaming
shotgun loaded with buckshot...
the way Laura Hunt was murdered night before last.
- Did I? - Yeah.
But he was really killed with a sash weight.
How ordinary. My version was obviously superior.
I never bother with details, you know.
I do. Well, so long.
Mind if I go with you?
- What for? - Murder is my favorite crime.
I write about it regularly...
and I know youll have to visit everyone on your l
shotgun
on one leg and leans against the wall. He wears a
headset... through which we hear the FBI guy yelling for
him.
This is JOHNNY UTAH.
BINOCULARS (FILTERED)
Utah, where the hell are ya!?
Utah takes his headset off...
??
INT. BANK
Pig Nose #1 LEAPS over the counter, holds a canvas sack
filled with booty from tellers drawers.
PIG NOSE #1
Fuckin shake it!
Pig Nose #2 nods with his snubby nose,
Shotgun shells, 12-gauge.
- Three shotgun shells, 12-gauge.
Hey, Divinci. Heard you got
a gang related, you lucky guy.
- Yeah, one less drugstore on the street.
- The worlds a safer place.
- Thanks.
- No problem.
Brown?
Blond?
Blond like Mel Gibson?
Want to go for a ride?
Why, did you get it?
As of last night, partner,
I have enough to make the down.
Picture me under some swaying palms,
sipping o
형광 표지된 염기(dNTP)를 흘려주며 사진 찍기를 반복하여 각 DNA strand들의 염기서열을 동시에 읽어낸다.
위에서 말하는 shotgun sequencing 또한 이와 같은 방법인 것으로 보인다. 예전에는 sanger sequencing에 대해서만 배웠는데, 차세대 기술들을 새로 접할 때마다 새삼 기술이 나날이 발전함을 체감하게 된다.
shotgun holes, is posted near a
pipeline support piling:
??
PIPELINE UTILITY CORRIDOR
PRIVATE PROPERTY
NO TRESPASSING
NO HUNTING
NO TRAPPING
NO SHOOTING
??
??
WIDER
??
??
Eric walks quietly past the sign, intent on something ahead
of him. Although still somewhat boyish in appearance, hes
confident and resolute in attitude. His clothes have a
distinctly western feel: Lucchese boots, Levis 501s, Ma
shotgun casually at his side, steps in front of them. He smiles laconically, and BLASTS OUT FIVE SHOTS that rip THROUGH US. By the second shot, the whole jungle blazes out with AUTOMATIC FIRE.
Out VIEW TURNS as the men around us are thrown and torn, screaming and scattering into the jungle. More AMERICANS appear; unexplainably, out of the growth. It is now that we fully SEE the bizarre manner in
shotgun wedding
Dad put family first
Cough up the money!
Thats for baby milk
Asshole!
Thought we were stupid?
I need my pinkie...
to play piano
Hes expelled
He was a useless father
Its hour to Toride
Then minutes to Ueno
on the Joban line
Tokyos a long way off
Mother left home after I began school
Then the divorce papers came
Who does she think she is?
But even in this crisis...
So what?
...was m