telomeres are disposable buffers blocking the ends of the chromosomes and are consumed during cell division and replenished by an enzyme, the telomerase reverse transcriptase.
Functions of Telomere
1. To protect chromosome ends from recombination,
fusion, and from being recognized as damaged
DNA
2. Allowing access to the telomerase enzyme to add
telomere repeats
3. Maint
telomere에 포함된 특정 서열의 DNA가 염색체가 분해되는 것을 막아준다는 사실, telomere DNA가 만들어지도록 하는 효소인 텔로머라아제를 발견하고 이를 통해 텔로미어와 텔로머라아제 효소 조절로 암과 노화를 예방하거나 조절할 수 있음 발견하였다.
이렇게 생명 연장의 핵심 기술로 부상하고 있는 텔
① University of Yonsei`s a biology research
- Used C.elegans for studying of telomere
② University of Leicester into medical`s Dr. Samany
- If population has dameged DNA ends, most of them is short-life
③ University of Southern Denmark`s Dr. Christensen
- Baby-faced person live longer than ordinary person
To make breakthrough progress in the molecular understanding of transcription and its regulation
disturbances in transcriptional regulation contribute cancer, inflammation, heart disease and metabolic diseases.
Essential to be able to direct this development to form only the wanted type of cells
Gene targeting in ES cell
1. ES cell culture
2. Construction of targeting vector
3. ES
1. DNA의존 RNA 합성
가. DNA 의존 RNA 폴리머레이즈(DNA-dependent RNA polymerase)
① DNA 의존 RNA폴리머레이즈는 본틀, Mg²⁺, NTP(UTP포함)이 필요.(Zn²⁺와도 결합함)
② dsDNA와 결합했을 때 가장 활성이 높다.
③ 개시를 하기 위한 시발체를 필요로하지 않는다.
④ 헬리케이즈 활성이 있어 이중