Abstract—We designed high-throughput microchip which detects single nucleotide polymorphisms (SNPs). The chip consisted of DNA extraction part and SNP detection part. DNA was extracted by lysis buffer, and then amplified by polymerase chain reaction (PCR). In SNP detection part, CEL I endonuclease selectively cleaved mismatched dsDNA, so wildtype DNA was distinguished from mutanttype. Sig
and xicS genes. Homologous recombination is type of genetic recombination which DNA sequences are exchanged between two similar or identical strands. It can be used for deletion of the target gene.
First, design and fabricate the similar DNA construct you want to insert into the chromosome in place of the wild-type allele. This constructs may contain selective marker that help isolate the kn
◉ Stringent Response
- Stringent Response란 세포가 서식하는 환경이 급격히 변하게 되거나 어떠한 요인에 의해서 세포의 생존에 위기가 닥쳤을 시에 이런 위급한 상황에 대처하기 위해서 세포내의 대사를 변화시키는 작용을 말한다. 이 작용을 통해 세포가 위기상황에서도 그 변화에 적응해 나가는 능력이
Abstract
Bioethanol is one of the alternative sources of oil resources which received attention with biodiesel. Many countries produce bioethanol from food sources such as corn and sugarcane, but it causes food-energy conflicts and farmland problem. So it needs to focus on new biomass which is sustainable, renewable, cost-effective and do not occurred any conflicts. Algae are one of the bioma