◈ Observation & Result
Ligased plasmid + 만든 CP Cell
Ligation + 상용 CP cell
Inserted plasmid + 상용 CP Cell
Plasmid only + 상용 CP cell
실험5 Subject Transformant 확인/ LB Broth에 (containing 100μg/ml of ampicillin) transformant를 multiplication
◈ Purpose
전날 만든 transformant가 제대로 배양되었는지 colony를 확인하고, ampicillin을 처리해
broth in his TV show and won in the ramen contest. His ramen was soon turned into products by Paldo. The award-wining kokomen sold more than 100 million packets in just over five months after it was released in August last year. It soon became unavailable in supermarkets, adding to its popularity.
The popularity encouraged many food companies to release various types of white broth ramen such a
Antibiotics acting on protein synthesis and other cytoplasmic process
Aminoglycosides (amikacin, gentamicin)
Tetracycline (tetracycline, minocyline)
Macrolides, Lincosamide, Ketolides, Streptogramin
(erythromycin, clindamycin, telithromycin, synercid)
Rifampicin
Chloramphenicol
Fusidic acid
Trimethoprim-sulphamethoxazole
Class
같은 계열의 항균제
Spectrum과 항균력이
제한효소에 의해 잘린 DNA들을 분리한다.
2. Principle
1) agarose gel electrophoresis 시 DNA의 이동에 영향을 주는 요인들
a. DNA 분자의 크기가 클수록 느리게 이동한다.
b. Agarose의 농도가 높을수록 느리게 이동한다.
c. DNA 형태에 따라 이동속도가 다르다. 일반적으로 supercoiled DNA > linear DNA > open circular DNA
d. 전압