(2) Active targeting
Nanoparticle passive targeting can be enhanced by ‘active targeting’, where specific ligands (peptides, antibodies, aptamers, or small molecules) are attached to the nanoparticle surface to bind receptors/antigens overexpressed on cancer cells. Targeting increases accumulation in tumors and lessens exposure to healthy tissue, which can improve a toxic drug’'s therape
imaging은 조사할 혈액을 많은 양 흘려보냄으로써 탐지 민감도를 향상시킬 수 있다. in vivo 유세포분석기(flow cytometer)를 이용한 CTC의 공초점 탐지는 2004년 처음 실행되었고 이동식 장치는 좀더 최근에 소개되었다. 그림 a에서 in vivo 유세포분석기에서 탐지된 혈중 GFP 양성 다발성 골수종 세포를 보여준다.
The cathode electrocatalyst was the electrostatic adduct of bilirubin oxidase (BOD) also a polyanion at physiological pH
Carbon Nanotube-AttachedGlucose Oxidase for Biofuel Cells
The carbon electrode coated with CNT–GOx composites
was applied as an anode in a model glucose/O2 biofuel cell
CNT-supported glucose oxidase (CNT–GOx) was examined in the presence of 1,4
cellular transport
- Microfilament is concerned with intracellular
transport of vesicles or other organelles
- Mitochondria transport in cells
Phalloidin method used in many actin detection
(since 1970)
Limitations
-less effective in live cellimaging
-Toxic effects on cell
Limitations
Photobleaching
Robust chromophores needed
Alexa, Seta or DyLight Flou
Image Study
Chest PA (4/17)
No newly developed lesion to suggest metastasis or active lung parenchymal disease
Abdomen Flat, Upright (4/17)
No abnormal gas, calcification, or mass shadow in the abdomen-pelvis
11. Diagnosis
Malignant melanoma stage IIIB(T4bN2bM0)
Metastasis to lymph node lower limb
12. P