Gene을 발현하는 중 ER과 GA에서 생산된 단백질에 당이나 지질을 붙이는 Post Translational Modification과정을 거치지 않는다. 따라서 동물 세포에서 생산되는 단백질과 같이 당단백질이나 당지질 등이 필요하게 되면 생산을 할 수 없다. 따라서 이런 식의 단백질은 많은 한계가 있다. 또한 Yeast는 정도가 크지는
Under the Hyperleptinemia condition
PPARα is increased during the rapid loss of fat
An Experiment of PPARα+/+ and PPARα-/- mice
→ Food intake and weight are reduced in both mice
but fat is reduced only in PPARα+/+
Under the Hyperleptinemia condition fat disappears in 7 days without any relation which is about quantity of free FA or ketone
→ Thus WAT is transformed to Fat b
b plasmid, FabG gene이 insert된 pET-14b plasmid, DDW, 10ⅹreaction buffer, 1ⅹTAE buffer, agarose, ethidium bromide, DNA ladder, DNA loading buffer,
pipette, binding column, spindown centrifuge, Water bath, microwave, Electrophoresis tank, e-tubes, ice box, gel cast, blade, heat block, vortex, table top centrifuge
5. Procedure
1) Digestion of DNA with Restriction Enzyme
① Extracted p