2. 2. Extraction
After the fermentation, the microorganisms(Alcaligenes latus) are filled with PHB in their body. These PHBs have to be separated from microorganisms to make final product. To separate PHBs from biomass, they are extracted from biomass by using solvent. These PHB extracted solvent will experience washing and drying process. After that, PHB can be precipitated as white powder.
like hydrogen fueled car. However, although our interest in fuel cell is increasing, the stage of the development is still slight. To develop fuel cell technique as the core industry, we have to construct effective infrastructures from extraction to supply. So, in this project, our team has searched the fuel cell technique dividing into four parts ; extraction, storing, type, merits & demerits.
extraction 시 사용되는 phenol의 pH : 7.9(RNA 시는 4.5)
Chloroform
Phenol이 작용시 적은 양이나마 수용액에 녹는다. 이렇게 녹은 phenol을 수용액에서 제거 및 Interphase가 잘 형성되게 해준다
Isoamyl alcohol
실험 시 거품이 생기는 거 방지 및 Interphase 형성 도움
Phenol : Chloroform : Isoamyl alcohol = 25 : 24 : 1 또는
Phenol :