culture is getting essential in our lives. As the meaning of culture have been changing from luxurious, expensive things into a part of normal life, it has gone with changes of leisure interests. A number of people make a plane to visit tourist attractions, romantic spots, or just near parks every weekend. Also, after an international tourism had been free in 1980's, tourists all around the world
culture and feel of a small company. Prior to joining Zappos, Tony co-founded Venture Frogs with Alfred Lin. Venture Frogs is an incubator and investment firm that invested in Internet startups, including Ask Jeeves, Tellme Networks, and of course, Zappos.com. Prior to Venture Frogs, Tony co-founded LinkExchange, an advertising network that was successfully sold to Microsoft for $265 million in 1
culture and feel of a small company. Prior to joining Zappos, Tony co-founded Venture Frogs with Alfred Lin. Venture Frogs is an incubator and investment firm that invested in Internet startups, including Ask Jeeves, Tellme Networks, and of course, Zappos.com. Prior to Venture Frogs, Tony co-founded LinkExchange, an advertising network that was successfully sold to Microsoft for $265 million in 1
es
Appeal
Culture Strength
Decision
Making
Ethical Decision Making Framework
4 You
Matrix Structure
George and Jones (2005), state that the matrix organizational structure
All Channel
Use same channel
All channel communication allows employees to communicate actively with each other, making all members free to contribute
Customer
Employee and customer are communicate each other
culture dish에 seeding한다.
(2) Virus infection
① 배지를 suction하여 제거한다.
② Virus stock이 포함된 배지를 고르게 뿌려준다.
200ul (바이러스) + 800ul(free FBS 배지) = 1ml
③ 한 시간 후에 stock이 포함된 배지 suction하고 PBS로 2회 washing한다.
④ PBS suction 후 10% FBS가 포함된 DMEM배지를 넣어준다.
⑤ 약 6시간 후