1. Heart disease & cardiac enzymes
1.1 heart disease
Team#5 has had researches about diagnosis of cardiovascular disease using cardiac marker (cardiac enzyme). Cardiovascular disease (heart disease) is a class of diseases that involve the heart or blood vessels (arteries and veins). Cardiovascular diseases remain the biggest cause of deaths worldwide, through over the last two decades, cardio
Marker for Myocardial Infaction”, 2010
Example : IMA급성심근경색증 diagnosis (acute myocardial infarction)
Requirement of ideal cardiac marker for IMA diagnosis
Existing in cardiac muscle - not any other parts of the body.
Fast emission when the cell is damaged.
Quantity of released markers should be in direct proprotion to size of enzyme.
Easy to assay, stable, and che
Markers of Allergic Inflammation
A vast amount of knowledge concerning the detection and treatment of allergic disease is derived, in part, from numerous in vitro studies investigating the parameters, mechanisms, and pharmacologic control of the inflammatory mediators released from basophils and mast cells. ........
Although histamine is most commonly measured following this IgE-mediated reac
marker로 사용한다.
5. 각각의 tube에 DNA loading buffer(1 μl)와 섞은 후, 각각의 well에 loading 한다. (DNA loading buffer는 agar에 가라앉히는 역할과 파란색 dye로 어디까지 내려왔는지를 알게 해주는 역할을 한다)
6. 75V 전압에서 전기영동을 하여, DNA가 충분히 분리되도록 한다(DNA의 경우는 인산기에 있는 negative charge