of probability of default (PD) estimates in internal ratings-based (IRB) capital requirements during benign credit conditions by using the PD estimates for a bank’s portfolios in downturn conditions. Addressing the same issue, the UK Financial Services Authority (FSA) has proposed an approach aimed at providing non-cyclical PDs in IRB requirements through the application of a scalar that conve
1. An outline of a nation
A. Characteristics of a nation
a. Language
Spain’s official language is Spanish (español). It is spoken by the vast majority of its people. Spanish has two major dialects—Andalusian and Castilian—which differ in their pronunciation of certain sounds. In a number of regions of Spain other languages are also important. Catalan, a relat
of what’s on her list or we’re in trouble (Lazarus).”
- EDLP (every day low prices) strategy and in-stock image stimulates purchasing year-round instead of primarily during the Christmas season
- baby diapers and formula are sold at or below cost, in hopes of winning over new parents and keeping them as customers as their children mature
- this strategy has won TRU a steadily increasing
of DNA with Restriction Enzyme
I. Apparatus
- Water bath, brief centrifuge
II. Reagents
- Plasmid, DDW, restriction enzymes [NdeI, BamHI], restriction enzyme reaction buffer
① NdeI: an endonuclease isolated from Neisseria denitrificans. In molecular biology, it is commonly used as a restriction enzyme.
② BamHI: a restriction enzyme, derived from Bacillus amyloliquefaciens. It has the
1. Date
Day 1 – 2008년 10월 27일 월요일
2. Subject & Purpose
Digestion of DNA with Restriction Enzyme & Agarose Gel Electrophoresis & Gel Purification
Plasmid pET vector와 FabG gene이 insert된 pET vector를 두 가지 제한효소, BamHⅠ과 NdeⅠ으로 자른 다음 전기영동을 통해 결과를 확인하고 Purification을 한다.
3. Principle
1) Restriction enzy