1. Organ bath study
Animal : New Zealand white rabbits coronary arterial (4-5mm in length)
Endothelium of the arterial ring was remove
0Ca2+-1mM EGTA sol. : KH sol. and addition of 1mM EGTA
2. Single-cell isolation and Ca2+ imaging
Stips loaded with 1uM fura-2/AM in Tyrode’s solution for 30min at room temperature
[R(F340/F380)], 510nm CCD camera
Dissociation constant of fluoresc