RNA isolation using Trizol (cell : in culture)
1.Using at least 106 cells, aspirate off the media and wash X1 with ice cold PBS (1-2 ml)
2.Aspirate off the PBS (Remove as much as possible) and add 1 ml of trizol
Alright so this is the world and there are five billion people on it. When I was a kid there were three. Its hard to keep up.
AMERICA FROM SPACE
The great continent through mist and swirling skies. (Satellites and other pieces of skycasting equipment float by.)
JERRYS VOICE
Thats better. Thats america. See, America still sets the tone for the world...
KID ON BASKETBALL COURT
A puberty-ravaged kid