Effect of NGF and inhibitors on neurite outgrowth of PC12 cells
We found that neurite outgrowth in the PC12 cells was stimulated by NGF and that ML-9, an Akt inhibitor, prevented this outgrowth.
Moreover NGF-induced neurite outgrowth was greatly enhanced in cells overexpressing a constitutive form of Akt, CA-Akt,
and dramatically inhibited in cells expressing a dominant negative Akt.