changes in market conditions induced Finland's farmers to switch from growing staple grains to producing meat and dairy products, setting a pattern that persisted into the late 1980s.
In response to the agricultural depression of the 1930s, the government encouraged domestic production by imposing tariffs on agricultural imports. This policy enjoyed some success: the total area under cultivation
Rusult
1.β 결정상 확인 (FT-IR)
PVDF/Clay 나노복합체의 β-결정상이 생성되었는지 확인하기 위해서 FT-IR을 이용하여 조사하였다. Figure를 보면 neat-PVDF에서 거의 나타나지 않았던 β-결정상 peak가 1275cm에서 CF peak를, 840cm에서 CF=CH peak를 보였으며, Clay 함량이 증가함에 따라 peak의 세기가 커지는 것으로 보아, β-
DISCUSSION
In a first test tube of water was used instead of catechol, PPO catechol even if there is a chain o-quinone melanin units to be created because no color change does not happen. In test tube three extracts were used instead of an A to extract B, extract B, extract A and the other thing is, tried to bath heated through. In other words, extract A was included in the PPO After getti
1.1.2. Cyclooxygenase-prostanoid cascade
Figure 1.1. Metabolism of arachidonic acid
Despite the diverse chemical structure of aspirin-like drugs, the antinociceptive effect of NSAIDs is mainly due totheir common property of inhibiting cyclooxygenases involved in the formation of prostanoids. Prostanoids areformed by most cells and act as autocrine and paracrine lipid mediators. They are n
PHB`s Separation & Purification
Target of this study conducted to obtain a purely material PHB purification process debris in the damaged cells, and to remove the remaining medium components. PHB has been removed during the refining process with just remove the present efficiency of the overall purification stayed in the 70-80%.
PHB Synthesis Pathway
Alcaligenes eutrophus PHB in normal circ
나) Sample 2.
물 : 아세톤 : MIBK = 15 : 15 : 30
RT
Area
부피(1차-3차)㎕
Acetone
1.245
12037926
1차
1.62911
2차
1.41867
3차
1.42526
MIBK
3.373
27977997
1차
5.06129
2차
4.99423
3차
4.93190
Water
0
0
1차
x
2차
x
3차
x
upper (extraction)
(검량선 1,2,3차식을 이용해 각각의 부피를 구한다.)
(sample2
HEK 293 EBNA1 cell
Freestyle medium
293 SFM II medium
Enriched medium (293 SFM II medium + 5X amino acid solution)
E.coli cell 안에서 형질전환 된 pTT
1. Freestyle midium에서 suspension 293 cell culture
2. Bioreator로 옮겨줌
3. DNA-PEI complex(pTT-EPO+PEI)로 tansfection 수행
4. 4시간 후 293 SFM II medium 첨가
5. 이틀 후 enriched medium에서 fed-batch culture
2] 선행연구
[ 항암제에 대한 암세포의 주요 내성기전 ]
1) 약물 배출 증가 (Increased efflux of the drug)
Cancer stem cells의 다약제내성(MDR:multidrug resistance)은 세포질로부터 항암 약물 을 밖으로 배출하는 Efflux pump가 과발현 되기 때문이다. 예를 들어, PGP (P-glycoprotein) 는 ATP 의존적인 Efflux protein
물질로서 눈으로 얼만큼 전기영동이 잘 일어났는지 볼 수 있도록 한다.)
Sample을 4min동안 끓여주고 spin down.
1)Gel 제작
Separating gel(5ml)
Stacking gel
H2O
1.9ml
H2O
2.1ml
30% acrylamide mix
1.7ml
30% acrylamide mix
0.5ml
1.5M Tris (pH8.8)
1.3ml
1.5M
아이들의 이상 여부를 확인했다. 그 결과 환경호르몬인 프탈레이트에 노출된 엄마들의 아이들일 수록 AGD의 길이는 짧았다. 프탈레이트는 프라스틱류에서 흘러나오는 환경호르몬 물질이다. 이것이 어떠한 경로를 통해서든 자궁 속 태아의 호르몬작용을 방해하여 남성화를 가로막고 있다고 말한다.