We have shown that in colonic smooth muscle, PKC-α and RhoA translocate and associate with each other in the particulate fraction in response to stimulation with acetylcholine.
We have also shown that RhoA interacts directly with PKC-α in vitro.
1) In human endothelial cells, activation of ROCK by RhoA requires PKC activation .
(1998,Hippenstiel S et al)
2) We have recentl
The purpose of the present study was to determine the role of RhoA prenylation in the activation of RhoA and in the maintenanace of the basal and stimulated (by U-46619) state, before and after prenylation inhibitor GGTI-297.
We also determined cellular distribution of RhoA and ROCK activity. For comparison, we used phasic rectal smooth muscle (RSM).
GGTaseI (=prenyltransferase) : Catalyz
7. 다 간 조직은 80ul 분주한 E-tube에 모아서 담근다.(ice-rac)
(전체 다 갈면 tapping 후 약 30s간 vortex->얼음, 녹을 때 까지, 상온에 노출 많이 X)
8. centrifuge (13.3rpm, 12min, 4℃) -> spectraphotometer ON
9. 샘플-> 얼음 상층액 60ul 떠서 E-tube에 분주 -> 마개 씌워 액체질소 통에 넣음
10. 남은 샘플-> 20ul 떠서 P.detc. sol.이