분야
pptx2.Background
3.Upstream
4.Midstream
5.Downstream
6.Price & Market
7.Conclusion
8.References
1,3-PDO is the monomer of polytrimethylene terephthalate.
It is widely used as the raw material of cosmetics, food, textile, lubricants, and medicines.
The yield of chemical method to produce 1,3-PDO is low, and there are many by-product by using chemical production of 1,3-PDO. As a result, it is difficult to produce 1,3-PDO by chemical method.
Using bioprocess, energy requirement is low, and the yield of process is high, so bioprocess can be used for commercial production of 1,3-PDO.
Recently, the technology that the production of highly concentrated 1,3-PDO is developed using Metabolic engineering. In this technology, recombinant E.coli is used by consuming glucose of glycerol in corn.
1,3-propanediol?
-Molecular Weight : 76.09g/mol
-colorless liquid
-density : 1.0597g/cm3
-water miscible
1. Biomaterials: environment-friendly materials
natural materials
2. Flexibility
3. Good flexibility at low temperature
4. Price competitiveness
5. Impossibility of natural synthesis
6. Production by microorganism
7. Use metabolic engineering
→ Development of technology to produce 1,3-propanediol
1,3-PDO is produced by fermentation of glycerol in recombinant E. coli
Selection organism: E. coli
-Reason : have rich set of genetic tools
closely related to the natural production of 1,3-PDO
2) Applied genetics: Strain development
-recombinant E. coli inserted regulon gene of K. pneumoniae dha
Production of high concentration of 1,3-PDO(135 g/L) from glucose using recombinant E. coli and Metabolic engineering
Midstream
: Cultivation in the bioreactor under proper condition
http://en.wikipedia.org/wiki/1,3-Propanediol
Metabolic engineering for the microbial production of 1,3-propanediol , Charles E Nakamura , Gregory M Whited,
Advances in biotechnological production of 1,3-propanediol Guneet Kaur, A.K. Srivastava , Subhash Chand
