and feeling the water seep
through your shirt
and getting through to your skin.
And the feel of the ground
growing soft beneath your feet
and the smell.
And the sound of the rain
hitting the leaves.
All the things they talk about
in the books that you havent read.
This is you.
Who would have guessed it?.
You.
Lesson .
Dialogue.
Catch this.
You give me the big one.
OK.
Dont you even...
So how come
A high AERIAL SHOT of the island features the Statue of Liberty prominently in the foreground then TRAVELS ACROSS the harbor, OVER the Battery and Lower Manhattan to Greenwich Village.
EXT. EAST 77TH STREET - DAY
A car is being hoisted up by a municipal tow truck while its owner is having a terrible screaming argument with a parking enforcement officer. DANA BARRETT comes home pushing a baby bugg
THE WILLIAM MUNNY KILLINGS
INT. ALICES ROOM - NIGHT
NIGHT IN ALICES ROOM. A little bit of moonlight coming in
through the tiny window might make a highlight here and
there but thats about all. Words begin to crawl across the
screen:
WRITTEN WORDS (crawl)
Of good family, albeit one of
modest means, she was a comely
young woman and not without
prospects. Therefore it was at
once heartbreaking and a
The Ox Bow Incident Script
Deader than a Paiutes grave.
Well?
- That guys awful slow getting there.
I feel sorry for him. Always in reach
and never able to do anything about it.
- I got a feeling she could do better.
- Youre boasting.
- Whatll you have? Whiskey?
- What you got?
Whiskey.
You ever see such a guy?
All winter Ive been thinking....
And all hes gots whiskey.
- Thats rotten, aint it?
-
The Unforgiven
Original Screenplay
by
David Webb Peoples
PRODUCTION DRAFT
April 23, 1984
INT. ALICES ROOM - NIGHT
NIGHT IN ALICES ROOM. A little bit of moonlight coming in
through the tiny window might make a highlight here and
there but thats about all. Words begin to crawl across the
screen:
WRITTEN WORDS (crawl)
Of good family, albeit one of
modest means, she was a comely
young woman and not w
and plump.
Yo, Osama! Osama! Get behind the register! Now!
- Did you hear what he said? - Move! Move!
- Please! Take it. Please. - Shut up, man. Come on.
- l want no trouble. - Shut the fuck up.
- Take it all. - Shut up!
- l just came to the country. - Dont you move.
- Do not move! - l want no trouble, sir. Take it all.
This is America, homeboy. Black people dont get what they want
- in this coun
LOST IN SPACE
by
Akiva Goldsman
CHAPTER 1: Earth 2058
---------------------
모
FADE IN ON:
EXT.-SPACE
PULL BACK slowly as MARS fills THE FRAME, a sphere of red desert and fast rushing crimson clouds.
A triangular CARGO SHIP descends from the dark of space.
PILOT (OVER)
Mars mining base, this is Grissom One,
Request final descent vector.
REVERSE ANGLE
EXT.-MARS
A row of giant red mountains and b
13 DAYS
DARKNESS. As the MAIN TITLES BEGIN, the theater thrums with
a subsonic HISS which mounts in all the rattling power of
THX, and we...
BURN IN, BRIGHT LIVING COLOR:
EXT. STRATOSPHERE - DAY
The glory of stratospheric dawn. The engines of a silver
Lockheed U-2F rasp upon the trace oxygen here at 72,500 feet.
Scattered cloud formations hang over the blue brilliance of
sea far, far below. In t
Abstract
실험에 사용되는 암세포(A 549)의 medium제조 와 Cell-thawing, Cell-culture의 실험방법에 대해서 알아보았다. 암세포를 배양할 때는 몸 안에 있을 때와 비슷하게 적절한 환경을 만들어 주어야한다. 암세포가 몸 안에서는 면역에 의해 균이 없는 상태에서 죽지 않고 자라지만 체외로 노출된 상태이므로
thawing을 해야 한다.Nitrogen tank에 있는 cell들은 Cryo media라는 특수 media를 사용한다. 여기서, cryo media는 complete media(DMEM) +10% FBS + 5% DMSO(Dimethyl sulfoxide(CH3)2SO).여기서 DMSO의 역할은 cryoprotectant의 역할을 한다. 즉, 세포가 천천히 얼게 만들어준다. Thawing을 할 때, water bath를 사용하여 media가 cell에 닿기 전에 따뜻