In catalyst, oxygen produced from H202
With sodium hydroxide, luminol Dianion is formed
The oxygen reacts with luminol dianion
This product is very unstable, so decomposes to 5-aminophathalic acid
As the exited state, 5-aminophathalic acid relaxes to ground state
Excess energy is emitted as blue light
Alkaline luminol, H2O2 solution is used on detecting NO2
The presence o
In synthetic luminol process we need luminol’s precursor. That is
5- nitrophthalhydrazide. It is synthesized by the reaction of 3-nitrophthalic acid
with hydrazine. This process is condensation reaction. First, hydrazine(N2H4) is
heated with the 3-nitrophthalic acid. This reaction is most conveniently carried
out in the high-boiling solvent triethylene glycol. To boil off excess water, t
The luminol is activated with an oxidant. Hydrogen peroxide solution and sodium hydroxide is
used as the activator. In the presence of an catalyst, the hydrogen peroxide is decomposed to form
oxygen and water. The iron in potassium ferricyanide is a catalyst for the reaction.
When luminol reacts with the sodium hydroxide, a dianion is formed. The oxygen produced from
the hydrogen peroxi
4.Enzyme electrode의 발전상황
1.글루코오스 센서
클라크(Clark)와 리온즈(Lyons)에 의해 처음으로 효소 전극의 원리가 제안되었다. 그들은 혈액 중의 포도당을 측정하기 위하여 글루코오스 센서의 원형을 제안하였다. 투석막 사이에 효소를 함유시켜 , 이 투석막을 혈액과 접촉시키면, 함유된 효소가 포도
화학적 환경조건에 영향을 받아 다시 반응하여 눈에 보이는 상태로 변화하는 과정으로 침강반응, 응집반응, 용균현상, 중화반응, 보체결합반응 등이 나타난다. 장기이식에서 보이는 거부반응도 그 일종으로 이러한 반응들은 감염증의 진단에 응용된다. 항원항체 반응시 나타나는 이러한 과정은 화학구