ACEinhibitor가 쓰이는데, 이번 과제에서는 ACE는 inhibitor와 어떤 mechanism으로 작용하는 것인지 molecular level에서 알아볼 것이며 참고 자료로 「Identification of Two Active Site Residues in Human Angiotensin I-converting Enzyme 」 Vol. 269, No.47, Issue of November 25 pp.29430-29434, 1994 THE JOURNAL OF BIOLOGICAL CHEMISTRY , 「The two homologous Domains of
of thromboxane A2( vasoconstriction)
further platelet activation, potential resistance to thrombolysis
2) → conformational change in the glycoprotein ⅡbⅢa receptor.
→high affirnity for the sequence arginine-glycine-aspartic acid on the fibrinogen gamma chain
→ platelet cross-linking and aggregation
B. coagulation cascade
acid) 100㎕를 가하여 4℃에서 1시간 동안 방치하였다. 그 후 증류수로 세척하고 실온에서 plate를 건조한 다음, 각 well에 1%(v/v)의 acetic acid에 녹인 0.4%(w/v) SRB용액을 100㎕ 첨가하고 상온에서 30분 동안 염색시켰다. 결합되지 않은 SRB 염색액은 1%의 acetic acid로 세척하여 건조하고 10mM의 Tris buffer 용액 100㎕를 첨
acid to prostanoid endproducts is carried out by cyclooxygenases (COX), also known as prostaglandin H synthase (PGHS), at two different sites on the enzyme.
Arachidonic acid is initially cyclized and oxidized to the endoperoxide PGG2 at the cyclooxygenase siteof the COX and the product then reduced to a second endoperoxide PGH2 at the peroxidase site. Subsequent formation of prostaglandin end
enzyme.
3) Our Group’s idea
a) Approach 1
Our first approach is to prevent the formation of complete hupL gene by mutating xisC gene. Functional XisC recombinase and HupL subunit will not be produced. Without the HupL subunit, there will be no functional uptake hydrogenase and the active site for converting hydrogen gas will not be present. The function of HupL is more crucial than Hup