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분야
pptxNuclear import
단백질 도식화
DLC-AS와 LC8과의 결합 여부
이종 DLC-AS와 NLS 융합 단백질
DLC-AS의 Nuclear localization 촉진
시간에 따른 단백질의 축적양
Gene therapy
Non-viral gene therapy
Gene therapy
References
- 성공률 낮음
- Immune response
- Toxicity
- Low transgenic capacity
- Genetic instability
- Insertional mutagenesis
- Virus‘s large size -> hard to diffuse
Gregory W. Moseley (2007). Dynein Light Chain Association Sequences Can Facilitate
Nuclear Protein Import. Molecular Biology of the Cell, 3024-3214.
Cohen, R. N., Rashkin, M. J., Wen, X., and Szoka, F. C., Jr. (2005). Molecular
motors as drug delivery vehicles. Drug Discov. Today Technol. 2, 111–-118.
Glover, D. J., Lipps, H. J., and Jans, D. A. (2005). Towards safe, non-viral
therapeutic gene expression in humans. Nat. Rev. Genet. 6, 299–-310.
Greber, U. F., and Way, M. (2006). A superhighway to virus infection. Cell
124, 741–-754.
Hubner, S., Xiao, C. Y., and Jans, D. A. (1997). The protein kinase CK2 site
(Ser(111/112)) enhances recognition of the Simian virus 40 large T-antigen
nuclear localization sequence by importin. J. Biol. Chem. 272, 17191–-17195.
Mastrobattista, E., Bravo, S. A., van der Aa, M., and Crommelin, D.J.A.
(2006a). Nonviral gene delivery systems: from simple transfection agents to
artificial viruses. Drug Discov. Today Technol. 2, 103–-109.
Mastrobattista, E., van der Aa, M., Hennink, W. E., and Crommelin, D.J.A.
(2006b). Artificial viruses: a nanotechnological approach to gene delivery.
Nat. Rev. Drug Discov. 5, 115–-121.
Pasdeloup, D., Poisson, N., Raux, H., Gaudin, Y., Ruigrok, R. W., and Blondel,
D. (2005). Nucleocytoplasmic shuttling of the rabies virus P protein requires
a nuclear localization signal and a CRM1-dependent nuclear export signal.
Virology 334, 284–-293.
Wagstaff, K. M., and Jans, D. A. (2006). Intramolecular masking of nuclear
localization signals: analysis of importin binding using a novel AlphaScreenbased
method. Anal. Biochem. 348, 49–-56.
