소개글
[화학공학] GRAM staining 그램염색 & observation of bacteria에 대한 자료입니다.
목차
1.Objective
2.Intro & Background
3.Apparatus & Regents
4.Procedures
5.References
본문내용
Keeping size and shape of cell
Preventing rupture of cells
by osmotic pressure
Hard because of Peptidoglycan
- 20 ~ 80mm : gram-positive
- 7 ~ 8 mm : gram-negative
24hours cultured
E.Coli in medium
Crystal violet
Gram iodine
95% ethyl-alcohol
Safranin solution
(25% safranin in 50% ethyl-alcohol)
Wash the slide glass with water quickly and blot dry gently.
Smear is now ready to be read with microscope. (Use oil immersion lens.)
참고문헌
http://food.chungkang.ac.kr/ybchoi/lecture/fmexp/fmbexp-10.htm
http://www.hitel.net/~U931018/text20ca.htm
http://www.hitel.net/~U931018/text20ox.htm
http://www.kccm.or.kr/default.asp
http://www.labbank.co.kr/Ajoo/product/ion_p1.asp
http://en.wikipedia.org/wiki/Gram_staining
Microbiology : A laboratory manual (James G.Cappuccino, Natalie Sherman)
http://www.enasco.com/product/SB0104M
http://safety.skku.edu
BROCK BIOLOGY OF MICROORGANISMS 3 EDITION Michael T.Madigan, p78 ~84
Willey 외, 일반미생물학(2008), 라이프사이언스
민철기,일반생물학실험서(1999), 라이프사이언스