domain are separated.
Zinc Finger Protein can recognize 9 base pairs.
ZFN can recognize 9 base pairs for each side by dimerization.
Specific sites can be cleaved by ZFN.
1. Promoter
Promoter is composed of
TF binding site and RNP binding site.
Strong promoter→Acceleration of TF binding →Acceleration of RNA polymerase binding
→Enhancing expression
binding
translocation activity.
divided functionally
into an amino terminal
domain(Hn) and a carboxyl
terminal domain(Hc).
(1) The carboxyl terminal domain
the binding domain.
tion of the toxin molecules
into cholinergic neurons.
(2) The amino terminal domain
the translocaion domain.
form ion channels in lipid bilayer
Structure The Heavy Chain
Structure The Light Chain
The ligh
①Entry(-> Host cell)
• Adenovirus의 숙주 세포 내로의 진입 시 Virus-host cell 사이의 2가지 interaction 존재
1. Host cell의 Receptor와의 interaction. -> Fiber protein의 knob domain
① CD46 for the group B human adenovirus
② the coxsackievirus adenovirus receptor (CAR)
**MHC1 & Sialic acid
2. The penton base protein then binds to the integrin family.
• 2
Immunoglobulin E
in health and disease
Basic characteristics of IgE
IgE is the antibody isotype that contains the ε heavy chain and it is a monomer with five domains in the immunoglobulin structure
in plasma
- concentration of less than 1 μg/mL
in serum
- a half-life of about 2 days
Basic characteristics of IgE
IgE binds to its receptor FcεR,
High-affinity receptors (FcεRI)
-wide var
domain receptor of the cytokine receptor family, which is found in many tissues in several alternatively spliced forms. The LEP gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.
명칭
Leptin
구성
16kDa Protein
분비 장소
Adipose Tissue(지방세포)
유전 정보 위치
사람 : 7번 염색체, 쥐 : 6번 염색체
역할
호르몬으로써 시
heme) 형성 장애에 의한 철 결핍성 빈혈(iron deficiency anemia), B12 엽산 결핍으로 인한 DNA 합성 결함으로 초래된 거대 적아구성 빈혈(megaloblastic anemia), 만성 질환의 빈혈(anemia of chronic disorders)과 골수 간세포 부전(marrow stem cell failure)으로 야기되는 재생불량성 빈혈(aplastic anemia), 순수적혈구무형성증(pure red cell
domain analysis
Disease gene mapping - splice variants, SNP
Public databases
UCSC, NCBI, Ensembl, …
Our own Programs
+
Noble Genes
III. In silico에서의 EST서열분석
- 최근 In silico 실험에서 사용하는 대표
적인 분석방식.
- 유전자비교를 통한 기능 해석
- EST의 이런 점을 이용 데이터베이스 구
축하여 In silico에서 사용
(1)EST(Expressed Sequan
hypersensitivity. Limited research has been conducted in this area, though some potential exists for a variant in the gene HSP70-HOM , whose protein is hypothesized to have direct involvement in the stimulation of an immune reaction to abacavir.
HIV VIRUS
Human Immunodeficiency Virus
RNA genome
Reverse Transcriptase
Glycoprotein gp120, gp41
CD4, chemokine receptor(CXCR4) binding
==Binding Theory==
1. 결속이론
: 서로 다른 NP 유형들이 문장에서 어디에 나타날 수 있는지에 대한 통사론적 제약에 관한 이론을 결속이론(Binding Theory)이라 부른다.
2. 결속이론과 관련된 3가지 명사의 개념
1) R-experssion(지시표현) : 세계에 존재하는 실재물을 지시함으로써 그 의미를 얻는 NP
☞ Proper names, Common
Party shall give the Disclosing Party prompt notice of such
request so that the Disclosing Party has an opportunity to defend, limit or
protect such disclosure; or
(d) is
established to be in the public domain other than as a consequence of a breach
of an obligation undertaken not to disclose the information; or
(e) is made
public by the Disclosing Party.
Article 4.TERM
The term of this