![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-1](http://images.happyhaksul.com/thumb/554/553577-0001.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-2](http://images.happyhaksul.com/thumb/554/553577-0002.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-3](http://images.happyhaksul.com/thumb/554/553577-0003.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-4](http://images.happyhaksul.com/thumb/554/553577-0004.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-5](http://images.happyhaksul.com/thumb/554/553577-0005.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-6](http://images.happyhaksul.com/thumb/554/553577-0006.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-7](http://images.happyhaksul.com/thumb/554/553577-0007.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-8](http://images.happyhaksul.com/thumb/554/553577-0008.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-9](http://images.happyhaksul.com/thumb/554/553577-0009.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-10](http://images.happyhaksul.com/thumb/554/553577-0010.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-11](http://images.happyhaksul.com/thumb/554/553577-0011.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-12](http://images.happyhaksul.com/thumb/554/553577-0012.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-13](http://images.happyhaksul.com/thumb/554/553577-0013.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-14](http://images.happyhaksul.com/thumb/554/553577-0014.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-15](http://images.happyhaksul.com/thumb/554/553577-0015.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-16](http://images.happyhaksul.com/thumb/554/553577-0016.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-17](http://images.happyhaksul.com/thumb/554/553577-0017.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-18](http://images.happyhaksul.com/thumb/554/553577-0018.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-19](http://images.happyhaksul.com/thumb/554/553577-0019.gif)
![[생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문)-20](http://images.happyhaksul.com/thumb/554/553577-0020.gif)
분야
pptx2.Actin imaging
3.Application
4.Summary
ATP bound actin polymerizes to form polar microfilament
Cell morphology, motility
Tightly regulated, coordinated by different actin-binding
proteins
3. Intracellular transport
- Microfilament is concerned with intracellular
transport of vesicles or other organelles
- Mitochondria transport in cells
Phalloidin method used in many actin detection
(since 1970)
Limitations
-less effective in live cell imaging
-Toxic effects on cell
Limitations
Photobleaching
Robust chromophores needed
Alexa, Seta or DyLight Flours
Minimized light exposure
Cell membrane permeability
Expression of transgenic protein
http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005744
http://zeiss-campus.magnet.fsu.edu/galleries/video/csu/videos/bsc1memeraldlifeactmcherrylysosomes/index.html
http://zeiss-campus.magnet.fsu.edu/galleries/static/cells/pl1ut2.html
