Detection of target proteins using fluorophore-conjugated antibodies.
Synaptophysin (red) and microtubule-associated protein 2 (MAP2; green) in mouse cortical neurons were fluorescently labeled with specific primary antibodies and Thermo Scientific DyLight 649-Conjugated Goat Anti-Rabbit or DyLight 488-Conjugated Goat Anti-Mouse Secondary Antibodies, respectively. Nuclei in both panels wer
Abstract—We designed high-throughput microchip which detects single nucleotide polymorphisms (SNPs). The chip consisted of DNA extraction part and SNP detection part. DNA was extracted by lysis buffer, and then amplified by polymerase chain reaction (PCR). In SNP detection part, CEL I endonuclease selectively cleaved mismatched dsDNA, so wild type DNA was distinguished from mutant type. Sig
SEM?
Scanning electron microscope
To analysis surface of specimen
Qualitative analysis at certain point
Operation principal of SEM
Emission electron from filament
Accelerate electron by electric field
Focusing electron by lens → mono-chromatic electron beam
Generate secondary electron and etc.
Detection of electron
detection, diagnosis and treatment of diseases
Detection and Diagnosis
Lab on chips help detection and diagnosis of diseases more efficiently
Nanowire and cantilever lab on chips help in early detection of cancer biomarkers
Receptor-mediated endocytosis of the nanocarrier(1)
Exocytosis of the nanocarrier(1a)
Dissociation, acidification, release of the drug into the brain; (1b)
detect and respond to security incidents. Where permissible, this may involve cross-border information sharing and cooperation.
Ethical standards and conducts: Participants should respect the legitimate interests of others.
Democracy: the security of information systems and networks should be compatible with essential values of a democratic society.
Risk assessment: Participants should c
Major cause of lower respiratory tract illness in infants and young children (up to 40% of pneumonia, 45% to 90% of bronchiolitis)
DIAGNOSIS
Viral culture: RVS grows well in several human cell lines, in which it causes formation of characteristic syncytia. Virus can be detected as early as 2 days and usually within 7 days on primary isolation from specimens collected from children.
A varie
detected by ELISA in INS-1 cells of control and treated with 33 mM glucose and/or CrCl3 for 48 hours. GSIS, glucose-stimulated insulin secretion
고 찰
1929년 Glaser 등8)은 brewer's yeast가 인슐린의 혈당강하효과를 돕는다는 것을 발견하였다. 또 1959년에는 Torula yeast를 사용한 실험에서 glucose intolerance를 나타낸다는 것을 발견하였다. 이
molecular signal(alamone)으로서, RNA polymerase에 직접 결합하여 promoter specificity와 transcription rate를 변화시켜 transcription을 조절하게 된다. (p)ppGpp는 RelA가 uncharged tRNA의 존재를 감지할 때, ribosome-associated synthetase(RelA)에 의해 만들어 지고, hydrolase인 SpoT에 의해 분해된다. 미생물의 활동에서 보면, amino acid가 생산되
detection limit = 10^-12 g for analyte molecules( according to Sauerbrey’s equation )
S A W
Surface Acoustic Wave sensor in which the acoustic wave on the surface of the piezoelectric crystal, launched and received on the same surface of the crystal.
The range of resonance frequency= 200~400 MHz
The detection limit of SAW sensors = 10^-15 g for analyte molecules
f0 = frequ