Gram의 시도
폐렴으로 사망한 환자의 조직에서 세균 염색
Crystal violet으로 염색-Lugol’s iodine 매염-ethanol 탈색
색이 남아있는 것(Gram-positive), 바랜 것 (Gram-negative)로 구분
당시 세균 염색 : Paul Erlich, alkaline aniline 염색법
수년 후, 독일 병리학자 Carl Weigart 대조염색으로 safranin 사용
1921 Hucker’s modification
1. Purpose
실험을 통해 그람 염색에 의한 세균 감별 염색(differential stain) 방법을 익히고, 세균의 Gram 양성(보라색) 및 음성(적색)에 따른 분류와 염색성의 원리, 방법, 기본 지식을 이해할 수 있게 한다.
2. Principle of GramStaining
미생물을 분류하는 방법에는 여러 가지가 있다. 즉, 미생물의 형태나 모
실험목적
세균의 성장을 억제시키는 물리 화학적방법의 기전을 알아보고자 한다
원리
열에 대한 세균의 감수성은 용기, 세균의 밀도집도, 습도, pH 등 세균의 외적요인과 세균의 발육최적온도나 열저항성 구조물인 아포 유무에 의해 좌우된다. 열을 사용하여 세균을 멸균하는 방법은 건열법과 습열
PFCs(Perfluorinated Chemicals)
Manmade chemicals, synthetic
Resistant to heat, water, oil, grease & stains
Stable, extremely resistant to breakdown
Widely used in diverse applications
Economically useful
Electronegativity of Fluorine
Partial charges in the polarized C-F bond
C-F bond
: strongest bond in
organic chemistry
: high energy bond
Two most studied compounds
2) mammonism
Mommonism is a one of serious problem. It is the phenomenon that people think that they can do everything if they have money. However, through industrialization age, people recognized that we have more important thing than money. If we seek only money and follow rich person, it is obvious that our society may be full of dimness and people would do everything to earn money regardless
1. Introduction of the Entire Project
Project purpose
We will take one step more in addition to analyzing the financial status of each company, and make suggestions at the companies’ point of view, and strategies for each country’s market. Thus, further realistic implications and practical applications to the real market will be suggested after the three kinds of analyses are done.
Especi
Experiment #1: Contamination
Principle
미생물은 우리 눈에 보이지는 않지만 우리가 생활 하는 주위 곳곳에 살고 있다. 우리가 숨을 들이쉬고 내쉬는 공기와 우리가 일상적으로 만지고 사용하는 물체와 심지어 우리의 손에도 미생물은 존재한다. 이것은 실험을 할 때에 무균조작법(aseptic technique)의 중요성을 깨
grams. In addition to the paper, the fast food restaurant within 0.5 miles of a residence increase weight gain by 49 grams over 9 months, which over 10 years calculates to 650 grams. However, the industry influenced substantially larger to female students rather than compared to the effects on male students. Therefore, the location of a fast food restaurant considerably associates with the determ
1. Introduction
1) Definition of preservation and food-spoilage micro-organisms
Food preservation is the process of treating and handling food to stop or greatly slow down spoilage (loss of quality, edibility or nutritive value) caused or accelerated by micro-organisms. Some methods, however, use benign bacteria, yeasts or fungi to add specific qualities and to preserve food (e.g., cheese, wine