Keeping size and shape of cell
Preventing rupture of cells
by osmotic pressure
Hard because of Peptidoglycan
- 20 ~ 80mm : gram-positive
- 7 ~ 8 mm : gram-negative
24hours cultured
E.Coli in medium
Crystal violet
Gram iodine
95% ethyl-alcohol
Safranin solution
(25% safranin in 50% ethyl-alcohol)
Wash th
단 계
Understand “Gram staining”
Distinguish“Gram positive & negative”via Gram staining method
Introduction & Background
Hans Christian Gram(1853~1938)→ Bacteriologist in Denmark
In 1884 → Develop Gram Staining
Gram staining
Methoed of diffentiating bacterial species → Two large groups (Gram positive & negative) → Using crystal violet (triarylmetha
Biodiesel을 얻기 위해 microalgae 사용
Microalgae에서 Oil의 함량을 높여야 함
TAG의 함량을 높여야 함
1) TAG biosynthesis pathway 관련 효소를 유전자수준에서 control
2) Bioreactor로 algae의 생장속도와 이차대사산물생성속도를 조절
Red를 이용한 lipid 측정방법>
Ultrapure Nile Red powder was dissolved in DMSO
(1
stain과 eosin stain에 모두 친화성을 보이며 이 상태의 세포를 polychromatophilic erythroblast라고 한다. 결국 세포질은 점점 더 eosiniphlic해지고 orthochromatic erythroblast라고 한다. 이 orthochromatic erythrobast는 핵을 외부로 방출하고 circulation에 참여하면서 reticulocyte가 된다. 이 상태의 세포는 poly ribosomes의 reticular networks
stained 모습.
F. Wild-type의 10일 후 모습.
G. WUS돌연변이가 삽입된 CLV3::GUS/wus-1 배에서는 CLV3가 발현되지 않았다.
H. CLV3::GUS/wus-1 seedling 10일 후 GUS-stained 모습.
I. wus-1 seedling 10일후.
J. STM의 돌연변이가 삽입된CLV3::GUS/stm-11 배에서는 CLV3가 약간 발현되는 것을 볼 수 있다.
K. CLV3::GUS/stm-11 seedling 1
Ⅱ. 세균의 분류
1) 모양에 의한 분류
현미경을 이용한 모양으로는 구균(cocci), 간균(bacilli), 나선균(spiral forms), filamentous 등이 있다. 염색법으로는 Gram staining, acid fast staining, flagella staining, capsule staining, endospore staining 방법이 있다.
2) 유전학적인 분류
DNA homology, rRNA sequence homology, plasmid, ....
Ⅳ. 세
stained with Wright’s-Giemsa showing >5% eosinophils, >1% basophils, and/or >50% goblet/epithelial cells suggest allergic disease of respiratory tract. Does not correlate with blood eosinophilia. Large numbers of neutrophils suggest infection. Both eosinophils and neutrophils suggest chronic allergy with superimposed infection. Significant number of false-positive and false-negative result occu
the First World War, Coca‐Cola’s red color has standized. Then that red color is using still for more than 50 years…”(from Coca‐Cola Korea Official Homepage) Red color, spencer fonted logo, contour bottle, etc..they were Coca‐Cola’s main characters and they are still. Consistency seems the main personality of Coca‐Cola which is distinguished from others.
1. BIOGRAPHICAL INFORMATION
Avi was born in New York City on December 23, 1937 and was raised in Manhattan. (Telgen, 71:7) When he was young, he had dysgraphia, which is a writing problem. This played an important role in his life. Most of his school papers were covered in red pen. Avi once said," I wasn't even paying attention (to the red marks) to it. I liked what I wrote."(JRDA) He was als
TITLE SENTENCE
: After seeing a Millionaire and wife leave their mansion, a man sneaked into the mansion to steal jewelry.
백만장자와 아내가 그들의 저택을 떠나는 것을 본 후, 한 남자가 보석을 훔치기 위해 저택으로 몰래 들어갔다.
It was 7:30 in the evening.
저녁 7시 30분이었다.
Millionaire Eduard Arellano and his Wife Susan were late.
백