Quantification & Protein Determination
Purpose : Bradford method를 통해 단백질을 quantification하고 determination 해준다.
Principle:
• Bradfrod method:
M.bradford가 개발한 단백질 정량법. 단백질에 결합하는 색소인 쿠마시브릴리언트(Coomassie Brilliant)블루 G250이 단백질에 결합하면 그 극대흡수가 변화하는 것을 이용한
damage
- High Pressure for Plasma
- Power for Frequency
- Gas concentration
- Deposition : 100nm
Why we use Sputter?
- Good for high Melting point Material
- Thickness uniformity
- Positive adhesion
- But, law growth rate and step coverage
Patterning : Photo Lithography
(Clear than Shadow Mask)
Why Al is used for electrode?
(Especially Gate)
damaged
C: global brand may be seriously damaged, leading to loss of brand equity
Ipanema-Introduction
The most cosmopolitan and wealthy neighborhood.
Legendary beach, restaurants, discos, nightclubs and luxurious apartments.
Chic boutiques, art galleries, theatres and cinemas.
Measuring Impact on Revenue
Direct Factors
Duration
% of local population
Consistency among
prot
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(1) Sample Preparation
Principle:
• Extraction Buffer(=lysis buffer) : Tris, TritonX-100, NP-40, SDS 등 여러 가지 detergent들이 cell wall과 mitochondria membrane을 깨트려주고 아래의 표에 제시된 protease inhibitors들이 세포 안의 protease가 단백질을 분해하는 것을 막아준다. 추가적으로 phosphatase inhibitor를 넣어 우
Stem cell calssification
1. Based upon location
Embryonic stem cells
Somatic/ adult stem cells
2. Based upon function
Totipotent
Pluripotent
Multipotent
Unipotent
Reject Response
Traditional Immuno-repressents
Constant administration
Little engraftment after 3~4 weeks
Host immune system impaired
Vulnerable to Infection
↓Need brief immuno-re