분야
ppt2.What is GFP
3.Mechanism
4.Applications
5.Conclusion
Seeing is believing.
Seeing is achieving.
One of the most important methods in bio-imaging.
GFP[Green Fluorescent Protein]
In 1962, Green protein was purified from jellyfish by Osamu Shimomura
In 1992, Douglas Prasher reported the cloning and nucleotide sequence of wtGFP
In 1994, expression of the coding sequence of GFP in heterologous cells of E. Coli and C.
elegans by the lab of Martin Chalfie
In 1996, the crystal structure of a GFP was turned out
In 2008, Martin Chalfie, Osamu Shimomura and Roger Y. Tsien shared the Nobel Prize in
Chemistry for their discovery and development of the fluorescent proteins
β-Barrel structure
one β-sheet, alpha helix containing the
chromophore
Inward-facing sidechains induce cyclization
reactions in tripeptide Ser65–Tyr66–Gly67
(maturation)
The tightly packed barrel protects the
chromophore from quenching by water
